2~3 tiny leaves —> trim (thining)
Day 0: lighting is maintained at 50 PPFD with a photoperiod of 24h. Temperature is 20C. Seed tray is covered to ensure a high relative humidity. Day 1: A fertilizer is added 24 hours after sowing. (EC: 1200 uS/cm, ph: 5.8, T: 25C and 250 PPFD)
Day 2: remove humidity cover.
Day 3&4: Removing double seedlings
Day 6: Increasing watering frequency
Day 11: leaves are beginning to overlap. Transplant
Temperature for Lettus: 24 C Day/19 C Night
Water Temperature No higher than 25C, cool at 26C, heat at 24C
Relative Humidity minimum 50 and no higher than 70%
Carbon Dioxide 1500 ppm if light is available, ambient (~390 ppm) if not
Light 17 mol m-2 d-1 (PPFD) combination of solar and supplemental light
Dissolved Oxygen (DO) in the Water: DO levels should be greater than 4 ppm (parts per million) to prevent growth inhibition
Ph to 6.4 for seedlings or 6.0 for mature plants.